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Evidence Guide: MSL975033 - Perform tissue and cell culture techniques

Student: __________________________________________________

Signature: _________________________________________________

Tips for gathering evidence to demonstrate your skills

The important thing to remember when gathering evidence is that the more evidence the better - that is, the more evidence you gather to demonstrate your skills, the more confident an assessor can be that you have learned the skills not just at one point in time, but are continuing to apply and develop those skills (as opposed to just learning for the test!). Furthermore, one piece of evidence that you collect will not usualy demonstrate all the required criteria for a unit of competency, whereas multiple overlapping pieces of evidence will usually do the trick!

From the Wiki University

 

MSL975033 - Perform tissue and cell culture techniques

What evidence can you provide to prove your understanding of each of the following citeria?

Maintain records

  1. Maintain records of batches of media
  2. Maintain records of active and stored tissue and cell lines
  3. Complete records so that they are retrievable, legible and accurate
  4. Ensure records conform to the information management, records, quality system and legal requirements
Maintain records of batches of media

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Maintain records of active and stored tissue and cell lines

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Complete records so that they are retrievable, legible and accurate

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Ensure records conform to the information management, records, quality system and legal requirements

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Interpret and schedule production requirements

  1. Review client request and confirm quantity and nature of cells, tissue or products
Review client request and confirm quantity and nature of cells, tissue or products

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Select appropriate media, materials, equipment and methods

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Plan parallel work sequences to optimise production

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Maintain a chain of custody, traceable to the worker, for all cells and tissues

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Work safely according to the legal and regulatory framework

  1. Identify hazards and workplace control measures associated with the sample, preparation methods, reagents and equipment
Identify hazards and workplace control measures associated with the sample, preparation methods, reagents and equipment

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Select, fit and use personal protective equipment (PPE)

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Address hazards and incidents as they arise

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Safely dispose of biohazardous materials and other laboratory waste

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Assemble and maintain tissue culture equipment

  1. Assemble, sterilise or decontaminate equipment according to workplace procedures
Assemble, sterilise or decontaminate equipment according to workplace procedures

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Perform pre-use and safety checks in accordance with relevant workplace and operating procedures

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Identify faulty or unsafe components and equipment and report to appropriate personnel

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Decontaminate area and equipment after use

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Prepare and test cell and tissue culture media

  1. Confirm media specifications and processes/methods
Confirm media specifications and processes/methods

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Prepare culture media to suit client request

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Sterilise culture media and check for sterility

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Perform quality control checks to ensure that culture media is fit for purpose

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Store culture media in accordance with specifications

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Obtain, monitor and maintain tissue and cell lines

  1. Retrieve/obtain the cell lines or tissue sample from fresh or preserved sources and prepare a culture
Retrieve/obtain the cell lines or tissue sample from fresh or preserved sources and prepare a culture

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Select specified culture media and add any necessary growth agents or nutrients

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Incubate cells/tissue in specified conditions

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Inoculate the media with the specified amount of sample

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Monitor growth of tissue and cell lines and products

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Detect contamination/abnormalities and troubleshoot materials, equipment and techniques

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Passage samples by subculturing to preserve or grow the line

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Harvest cells or cell products to optimise yields

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Preserve cells and tissues

  1. Select the appropriate preservation method
Select the appropriate preservation method

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Preserve the cell lines or tissue in accordance with the method

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Check preserved cell lines regularly to ensure viability is maintained

  1. Maintain records of batches of media
Maintain records of batches of media

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Maintain records of active and stored tissue and cell lines

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Complete records so that they are retrievable, legible and accurate

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Ensure records conform to the information management, records, quality system and legal requirements

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Assessed

Teacher: ___________________________________ Date: _________

Signature: ________________________________________________

Comments:

 

 

 

 

 

 

 

 

Instructions to Assessors

Required Skills and Knowledge